Publications

"We performed live cell imaging using the 24-channel microscope zenCELL owl. This allowed us to monitor the different migration behaviour of cells in real time. Before starting cell monitoring, the culture medium was exchanged by fresh culture medium containing our target treatments. Cell motility was observed over 24 hours at 37°C and 5% CO2 using the zenCELL owl system with the following settings: total time lase imaging: 24 hours, nterval: 10 minutes, exposure: 4, gain: 1, illumination: 43%, brightness: 58%." (p.29)

"Immediately afterwards, monitoring began with a 24-channel

microscope in the incubator, and 1 image per hour was acquired (zenCell owl, innoME GmbH, Espelkamp, Germany). Cells were left to settle for 24 h before Xevinapant was added in different concentrations. Then, 3 h after the application of Xevinapant, cells were irradiated with a dose of 2 Gy. After 48 h of treatment with Xevinapant, the medium was replaced with drug-free medium." (p.3)

"3 × 105/2000 μL cells were seeded into each well of 6-well sterile plates and cultured in an incubator for 24 h to adhere to the base and grow. Cells, whose medium was changed after washing with PBS twice, were monitored taking into account the scheduled times for the study (24h for qPCR, 48 h for wound assay using Zencell System)." (p.273)

"To obtain a time course of intoxication, the zenCELL owl 24 channel automated microscope (innoME GmbH, Espelkamp, Germany) was used. Images were obtained every 10 min for 8–12 h." (p.11)

"To evaluate how the cells interact with SPIONs at different  concentrations, we monitored them under a 24-well microscope (zenCELL  owl; innoME GmbH, Espelkamp, Germany). Cells were seeded in a 24-well  plate with 30,000 cells and 0.8 mL of medium per well. Immediately  afterwards, monitoring began with a 24-well microscope in the incubator, which acquired one image every hour or quarter of an hour." (p.3)

"Unbiased analysis of time-lapse data using zenCELL owl built-in algorithms allowed continuous estimation of cell coverage and detachment, although it could not be trained to recognise the elongated phenotype. As expected, cell coverage increased with time under control conditions, while the proportion of detached cells remained constant at

approximately 5%." (p.7)

''The views of each well in the plate were recorded through a live-cell dynamic imaging and analysis system (zenCELL owl) every 10 min for 36

hours. In the recorded images, B16F10 tumor cells were fusiform, and macrophages were round, making them distinguishable. Observing

the video formed by playing the images continuously, round macrophages were in groups attacking the single fusiform B16F10 tumor cell, and this cell cluster was regarded as a phagocytosis count. Phagocytosis cell cluster counts of all groups were quantified every 5 hours." (p.14)

''The Dox solution was diluted with new medium to the concentration of 100 nM, 250 nM, 500 nM, or 1000 nM, and the new medium was replaced after 24 hours, and placed it on the zenCELL owl, a live cell dynamic imaging and analysis system (zenCELL owl device, InnoME GmbH, Germany), to observe for 5 days, and each picture was taken at per-hour intervals." (p.341)

''siRNA-transfected AsPC-1 cells were seeded at low density(2 × 10⁴) in 24-well plates in triplicate (in duplicate for control siRNA) after which cell growth was monitored in real time for 8 days in a zenCELL Owl live cell imaging microscope (innoME GmbH, Germany). Images were collected at 20 min intervals and the equipment software calculated the percentage of confluence of adherent cells at each data point [51]." (p.484)

''The plates were placed into an incubator at 37 °C, and cell migration was monitored in real-time by micro-cinematography [...]. The extent of cell migration at 6, 24 and 36 h was expressed as the wound width of the scratch relative to the initial scratch, expressed as %." p.4)

''This experiment revealed that remdesivir decreases the cell number (12.5 μM; Fig. 1e). Live cell microscopy suggested that the reduced cell number of H9c2 cells treated with remdesivir relative to untreated controls was due to inhibition of cell proliferation rather than induction of cell death (Supp. Videos, Fig. S1a)." (p.2347)

''Live-cell imaging was performed with a 24-channel incubator microscope (zenCELL owl; innoME GmbH, Espelkamp, Germany) at 37 °C and 5% CO2. Cells and HA-SH were prepared and seeded as previously described in a 24-well plate and subsequently imaged every 5 min for 48h. A digital phase contrast was applied using the zenCELL owl software 3.2." (p.12)

''In addition, a total of 1 x 10⁴ cells were seeded per well in a 24-well plate format under standard growth conditions at 37°C, the total cell number over time was assessed by live cell imaging during 120 hours using the zenCELL owl incubator microscope and proprietary zenCELL owl software, acquiring images every 20 minutes (innoME GmbH)." (p.688)

''Subconfluent cells were treated with either 10 ng/mL mycolactone, DMSO equivalent to mycolactone dose, 100 ng/mL IL-1β or 400 ng/mL LPS for 24 hrs or as indicated in figure legends. For real-time imaging, endothelial cells were plated onto 24-well plates overnight, treated as indicated and imaged every 30 minutes by zenCELL Owl incubator microscope [...] for 24 hrs. Time lapse videos were generated with zencell-owl software (version 3.3, innoME GmbH)." (p.24)

''U87 cells were seeded on collagen coated vs non-coated 24-wells in a density of 30.000 cells. The media was enriched with and without free floating fibers in a density of 1 mg/ml. After 2 h 24-well plate were placed into a zenCELL owl life cell imaging microscope [...] for 3 days. Wells were recorded every 17 min and cell coverage, number of adherent cells, number of non-adherent cells and total number of cells for each single culture well was monitored. Diagrams and videos of life cell imaging were acquired using zenCELL owl [...]." (p. 4)

''In order to follow the motility of Paramecium under the different chemical conditions, the cell culture plates were placed into a zenCell Owl incubator microscope apparatus (innoME GmbH), that is compatible with the standard culture vessels. The device is equipped with 24 independent miniaturized cameras, thus it is capable of the automated, simultaneous monitoring of the 24 cell cultures with real time data capturing and visualization on PC. [...] Paramecium cultures were monitored for 24 h with 5 min of recording intervals.'' (p.3)

''An aquatic toxicological model system was developed to test the remediation efficacy of the aerogels containing living bioindicator cultures (Paramecium caudatum). The motility of paramceia proportional to thier viability was monitored by time-lapse video micrscopy (zenCell Owl incubator micrscope). The viability of paramecia was quantified by analyzing the pixel differences of sequential images by Image J software. The survival time of paramecia was estimated by fitting a linear trendline on the data points'' (p. 5-6)

''H9C2 cardiomyocytes in the logarithmic phase were used in this

assay. The cultured cells were automatically monitored and imaged

using a zenCELL owl device (InnoME GmbH, Germany), a live cell

monitor with independent mini-microscopes. Cell Count Kit-8 (CCK-8)

was used to evaluate cell viability.'' (p. 3)

''MG-63 cells seeded in 24-well plates (3x10^4 cells/well/450 µL) were treated with the treatments shown in Table 1 to investigate the morphological and surface adhesion changes of vitamin D, atorvastatin and TNF-alpha on osteosarcoma cells. For 48 hours, real-time images of the cells were monitored and recorded with a zenCELL owl microscope in 1-hour cycles (Figure 18-21).'' (p.74)