Publications with the zenCELL owl

*all rights reserved to the authors & the publishers

Annotation and functional characterization of long noncoding RNAs deregulated in pancreatic adenocarcinoma



''siRNA-transfected AsPC-1 cells were seeded at low density(2 × 104) in 24-well plates in triplicate (in duplicate for control siRNA) after which cell growth was monitored in real time for 8 days in a zenCELL Owl live cell imaging microscope (innoME GmbH, Germany). Images were collected at 20 min intervals and the equipment software calculated the percentage of confluence of adherent cells at each data point [51]." (p.484)

 

Acidic ascites inhibits ovarian cancer cell proliferation and correlates with the metabolomic, lipidomic and inflammatory phenotype of human patients



''The plates were placed into an incubator at 37 °C, and cell migration was monitored in real-time by micro-cinematography [...]. The extent of cell migration at 6, 24 and 36 h was expressed as the wound width of the scratch relative to the initial scratch, expressed as %." p.4)

 

The potential of remdesivir to affect function, metabolism and proliferation of cardiac and kidney cells in vitro



''This experiment revealed that remdesivir decreases the cell number (12.5 μM; Fig. 1e). Live cell microscopy suggested that the reduced cell number of H9c2 cells treated with remdesivir relative to untreated controls was due to inhibition of cell proliferation rather than induction of cell death (Supp. Videos, Fig. S1a)." (p.2347)

 

Reinforced Hyaluronic Acid-Based Matrices Promote 3D Neuronal Network Formation



''Live-cell imaging was performed with a 24-channel incubator microscope (zenCELL owl; innoME GmbH, Espelkamp, Germany) at 37 °C and 5% CO2. Cells and HA-SH were prepared and seeded as previously described in a 24-well plate and subsequently imaged every 5 min for 48h. A digital phase contrast was applied using the zenCELL owl software 3.2." (p.12)

 

Tumor targeting with bacterial Shiga toxin B-subunit in genetic porcine models for colorectal cancer and osteosarcoma



''In addition, a total of 1 x 104 cells were seeded per well in a 24-well plate format under standard growth conditions at 37°C, the total cell number over time was assessed by live cell imaging during 120 hours using the zenCELL owl incubator microscope and proprietary zenCELL owl software, acquiring images every 20 minutes (innoME GmbH)." (p.688)

 

Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1β, are linked to fibrin formation in Buruli ulcer lesions



''Subconfluent cells were treated with either 10 ng/mL mycolactone, DMSO equivalent to mycolactone dose, 100 ng/mL IL-1β or 400 ng/mL LPS for 24 hrs or as indicated in figure legends. For real-time imaging, endothelial cells were plated onto 24-well plates overnight, treated as indicated and imaged every 30 minutes by zenCELL Owl incubator microscope [...] for 24 hrs. Time lapse videos were generated with zencell-owl software (version 3.3, innoME GmbH)." (p.24)

 

PCL micro-dumbbells – A new class of polymeric particles reveals morphological biofunctionality



''U87 cells were seeded on collagen coated vs non-coated 24-wells in a density of 30.000 cells. The media was enriched with and without free floating fibers in a density of 1 mg/ml. After 2 h 24-well plate were placed into a zenCELL owl life cell imaging microscope [...] for 3 days. Wells were recorded every 17 min and cell coverage, number of adherent cells, number of non-adherent cells and total number of cells for each single culture well was monitored. Diagrams and videos of life cell imaging were acquired using zenCELL owl [...]." (p. 4)

 

In situ remediation efficacy of hybrid aerogel adsorbent in model aquatic culture of Paramecium caudatum exposed to Hg(II)



''In order to follow the motility of Paramecium under the different chemical conditions, the cell culture plates were placed into a zenCell Owl incubator microscope apparatus (innoME GmbH), that is compatible with the standard culture vessels. The device is equipped with 24 independent miniaturized cameras, thus it is capable of the automated, simultaneous monitoring of the 24 cell cultures with real time data capturing and visualization on PC. [...] Paramecium cultures were monitored for 24 h with 5 min of recording intervals.'' (p.3)

 

Modelling of heavy metal accumulation in aquatic ecosystems, development of selective sorbents for metal ion sequestration



''An aquatic toxicological model system was developed to test the remediation efficacy of the aerogels containing living bioindicator cultures (Paramecium caudatum). The motility of paramceia proportional to thier viability was monitored by time-lapse video micrscopy (zenCell Owl incubator micrscope). The viability of paramecia was quantified by analyzing the pixel differences of sequential images by Image J software. The survival time of paramecia was estimated by fitting a linear trendline on the data points'' (p. 5-6)

 

Ophiopogonin D alleviates diabetic myocardial injuries by regulating mitochondrial dynamics



''H9C2 cardiomyocytes in the logarithmic phase were used in this

assay. The cultured cells were automatically monitored and imaged

using a zenCELL owl device (InnoME GmbH, Germany), a live cell

monitor with independent mini-microscopes. Cell Count Kit-8 (CCK-8)

was used to evaluate cell viability.'' (p. 3)

 

Effects of statins & Vitamin D on osteosarcoma


Mrs. Betül Özbek Iptec
2019, Ankara Yilidirm Beyazit University, Turkey

''MG-63 cells seeded in 24-well plates (3x10^4 cells/well/450 µL) were treated with the treatments shown in Table 1 to investigate the morphological and surface adhesion changes of vitamin D, atorvastatin and TNF-alpha on osteosarcoma cells. For 48 hours, real-time images of the cells were monitored and recorded with a zenCELL owl microscope in 1-hour cycles (Figure 18-21).'' (p.74)

 
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