Buy 48-Well plate - TC-treated and sterile | minimalistic screening assay by innoME
48-well cell culture plates for miniaturized drug screening, cytokine assays, and dose-response experiments – TC-treated, sterile, USP Class VI polystyrene
The innoME 48-well plates offer 0.95 cm² of growth area per well, providing an efficient mid-size format between 24-well and 96-well plates—ideal for miniaturized drug screening protocols, cytokine assays, and dose-response studies with multiple concentration levels. The low medium volume of 0.19 to 0.48 mL per well conserves expensive reagents.
Made from USP Class VI medical-grade polystyrene — gamma-sterilized (SAL 10⁻⁶), endotoxin-free, DNase-free, RNase-free. TC-treated surface for optimal cell adhesion. The 48-well format is particularly valuable when more conditions are needed than the 24-well format can accommodate, but the volume of the 96-well format is too small.
Anwendungen der 48-Well Platte
Miniaturized Drug Screening
48-well format for dose-response studies with 8 concentrations × 6 replicates—all in a single plate. Cost-effective for expensive drug candidates: 0.3 mL/well instead of 0.7 mL (24-well) or 3 mL (6-well). Compatible with luminescence and fluorescence plate readers.
Cytokine & Chemokine Assays
48-well format for cytokine stimulation experiments with multiple concentrations: IL-2, TNF-α, IFN-γ, TGF-β dose-response curves. Supernatant collection (100–200 µl) for ELISA without disrupting the culture. Sufficient cells for downstream analyses (flow cytometry, Western blot).
Antibiotic susceptibility testing
Miniaturized antibiotic assay on cell lines: MIC determination in a 48-well format — more concentration levels than in a 24-well format, more cell material than in a 96-well format. Standard for cytotoxicity testing of antimicrobial peptides and antibiotics according to ISO 10993-5.
siRNA / shRNA Screening
48-well format for RNAi screening with multiple target genes: Each transfection condition is performed in duplicate or triplicate, providing sufficient cell material for qRT-PCR and Western blot. More cost-effective than the 24-well format when reagent consumption needs to be minimized.
Proliferationsassays
48-well format for BrdU, EdU, Ki67, and MTT proliferation assays under various stimulation conditions. Direct fixation and staining in the well—easier than trypsinization for flow cytometry.
Primary neuronal cultures
Primary neuronal cultures require coated surfaces (poly-L-lysine, laminin) and a small volume to ensure optimal nutrient concentration. 48-well plates with 0.3 mL of Neurobasal medium—ideal for electrophysiological stimulation experiments with a limited number of primary cells.
Technical Specifications
Number of wells
48 Wells (6×8 Anordnung)
area for expansion
0,95 cm² pro Well
Recommended volume
0,19 – 0,48 ml pro Well
Well diameter
11,0 mm
Soil type
Flat Bottom
Material
USP Class VI polystyrene (PS)
Surface
TC-treated (vacuum plasma)
Sterilisation
Gamma-irradiated, SAL 10⁻⁶
Endotoxin
Endotoxin-free, pyrogen-free
Lid
With lid, ventilated
Colour
Transparent
Packaging
1 tray/bag, sterile packaging
Which format is the right one?
Vergleich aller innoME Multiwell-Plattenformate — das 48-Well ist hervorgehoben:
| Format | Growth area/wave | Rec. Vol/Wave | Typical application |
|---|---|---|---|
| 6-Well | 9.5 cm² | 2.2–4.5 ml | Primary cells, organoids, transfection |
| 12-Well | 3.8 cm² | 1.2–1.9 ml | Transfection, luciferase, differentiation |
| 24-Well | 1.9 cm² | 0.55–0.88 ml | TEER, Drug Screening, Scratch Assay |
| 48-Well | 0.95 cm² | 0.19–0.48 ml | Miniaturised screening, Cytokines |
| 96-Well | 0.32 cm² | 0.1–0.2 ml | HTS, ELISA, Proliferation assays |
Why choose innoME 48-well plates?
USP Class VI medical polystyrene – highest purity, no cytotoxicity
Gamma-sterilised (SAL 10⁻⁶) – endotoxin-free, DNase-free, RNase-free
TC-treated surface – optimal cell adhesion for adherent cell lines
SBS/ANSI-compliant format – compatible with all plate readers & robots
Available from stock – fast availability, no minimum order quantity
Compatible with zenCELL owl – Live cell imaging directly in the plate
FAQ – 48-Well Platten
A 48-well plate is ideal when: (1) more than 24 conditions need to be tested, but (2) the volume of a 96-well plate (0.1–0.2 mL) is too small for downstream analysis. Example: 8 drug concentrations × 6 replicates = 48 wells—in a single plate, with enough cell material for Western blotting.
Typical: 2–5 × 10⁴ cells/well for fast-growing cell lines. For primary cells and neurons: 1–2 × 10⁴ cells/well. Maximum capacity: approximately 3–5 × 10⁵ confluent cells/well. Seed 24 hours before the experiment to allow for adhesion.
Yes — 100–200 µl of supernatant from the 48-well plate is sufficient for most ELISA kits (typical sample volume 50–100 µl). Collect the supernatant without disturbing the culture and store it at -80°C or measure it immediately.
Yes — 48-well plates have an SBS-compliant outer format and are compatible with all standard plate readers (Tecan, BioTek, PerkinElmer, BMG). For luminescence measurements: black walls recommended (available upon request). For absorption measurements: standard transparent plate.
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