Buy 6-well plates - TC-treated & sterile | disposable cell culture | innoME
Large-area 6-well cell culture plates for primary cells, organoids, virus production & transfection - TC-treated, sterile, USP Class VI polystyrene
The innoME 6-well plates bid with 9.5 cm² growth area per well Maximum space for demanding cell cultures - primary cells, organoids, large cell populations and transfection experiments. The recommended medium volume of 2.2 to 4.5 ml per well optimises the supply of nutrients and reduces the frequency of changes.
Made from USP Class VI medical-grade polystyrene - gamma-sterilised (SAL 10-⁶), endotoxin-free, DNase-free, RNase-free, no cytotoxicity. TC-treated surface for optimal cell adhesion of adherent cell lines. SBS-compliant external format for all plating robots and pipetting machines.
Applications of the 6-well plate
Primary cells
Primary cells require maximum growth surface and optimal adhesion conditions. The 6-well plate with 9.5 cm²/well is the standard format for primary cultures: hepatocytes, neurones, cardiac myocytes, PBMC, mesenchymal stem cells (MSC). TC-treated surface ensures uniform cell adhesion without additional coating.
Organoid cultivation
Organoids and 3D cell cultures require sufficient volume and surface area - the 6-well plate is the ideal format. Combination with Matrigel/Geltrex or Ultra-Low Attachment surface (available as a variant) for spheroid formation. Maximum volume of 4.5 ml/well enables sufficient extracellular matrix embedding.
Transfection & CRISPR
Standard transfection format for all common protocols (lipofectamine, PEI, calcium phosphate). 9.5 cm²/well provide sufficient cell mass for Western blot, flow cytometry and PCR analyses after transfection. CRISPR-Cas9 editing experiments benefit from the large growth area for single-cell cloning after selection.
Virus production
Lentiviral, adenoviral and AAV vector production in HEK293T cells - 6-well plate is the standard small-scale format for virus production. Sufficient volume for calcium phosphate transfection (500 µl precipitate + 2 ml medium). Non-TC variant on request for suspension cell lines.
Wound Healing Assay
6-well format for manual scratch assay and light-based wound healing with innoME's Scratch Maker. Compatible with zenCELL owl Live Cell Imager for continuous real-time monitoring of wound healing - without manual time point measurements. Coated version (Coating Plates) available for optimised cell adhesion.
Western blot preparation
Sufficient cell material for protein extraction for Western blotting: Typically 200-500 µg total protein from a confluent 6-well plate. Direct lysis in the well with RIPA buffer. Significantly more efficient than several small wells - one 6-well plate replaces approx. 6 × 12-well plates.
Technical Specifications
Number of wells
6 wells (2×3 arrangement)
area for expansion
9.5 cm² per well
Recommended volume
2.2 - 4.5 ml per well
Well diameter
34.8 mm
Soil type
Flat Bottom
Material
USP Class VI polystyrene (PS)
Surface
TC-treated (vacuum plasma)
Sterilisation
Gamma-irradiated, SAL 10⁻⁶
Endotoxin
Endotoxin-free, pyrogen-free
Lid
With lid, ventilated
Colour
Transparent
Packaging
1 tray/bag, sterile packaging
Which format is the right one?
Comparison of all innoME multiwell panel formats - the 6-well is highlighted:
| Format | Growth area/wave | Rec. Vol/Wave | Typical application |
|---|---|---|---|
| 6-Well | 9.5 cm² | 2.2–4.5 ml | Primary cells, organoids, transfection |
| 12-Well | 3.8 cm² | 1.2–1.9 ml | Transfection, luciferase, differentiation |
| 24-Well | 1.9 cm² | 0.55–0.88 ml | TEER, Drug Screening, Scratch Assay |
| 48-Well | 0.95 cm² | 0.19–0.48 ml | Miniaturised screening, Cytokines |
| 96-Well | 0.32 cm² | 0.1–0.2 ml | HTS, ELISA, Proliferation assays |
Why innoME 6-well plates?
USP Class VI medical polystyrene – highest purity, no cytotoxicity
Gamma-sterilised (SAL 10⁻⁶) – endotoxin-free, DNase-free, RNase-free
TC-treated surface – optimal cell adhesion for adherent cell lines
SBS/ANSI-compliant format – compatible with all plate readers & robots
Available from stock – fast availability, no minimum order quantity
Compatible with zenCELL owl – Live cell imaging directly in the plate
FAQ - 6-well plates
Typical sowing: 0.5-1 × 10⁶ cells/well for slow-growing primary cells, 2-5 × 10⁵ cells/well for fast-growing lines (HeLa, HEK293). Adjust confluence after 24-48h. The 9.5 cm² area holds up to approx. 5 × 10⁶ confluent cells (depending on cell line and size).
TC-treated (Tissue Culture treated): Vacuum plasma treatment makes the surface hydrophilic and negatively charged - for adherent cell lines. Non-TC: Untreated hydrophobic PS surface - for suspension cells or when a proprietary coating (collagen, fibronectin, Matrigel) is applied.
Yes - innoME's coated 6-well plates are directly compatible with the zenCELL owl Live Cell Imager and the Scratch Maker for Wound Healing Assays. The zenCELL owl end-to-end system (Scratch Maker + zenCELL owl + software) is specially optimised for 6-well and 24-well formats.
Recommended: 2.2 ml (minimum for complete soil coverage) to 4.5 ml (maximum without risk of overflow). Standard for most protocols: 2.5-3 ml. For transfection with calcium phosphate: 2 ml medium after addition of the precipitate.
Yes - Ultra-Low Attachment 6-well plates are available on request. These prevent cell adhesion through a special hydrophilic coating - ideal for spheroid formation, organoids and suspension cultures. Please contact us for availability and prices.
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